Table 1 PCR Primers Used in this Study
From: Mechanistic study of the cause of decreased blood 1,25-Dihydroxyvitamin D in sepsis
Genes | Forward primers | Reverse primers |
|---|---|---|
GHR | 5′-AGGTCTCAGGTATGGATCTTTGTCA-3′ | 5′-GCCAAGAGTAGCTGGTGTAGCCT-3′ |
IGF-1 | 5′-GCTTGCTCACCTTCACCAGC-3′ | 5′-AATGTACTTCCTTCTGAGTCT-3′ |
IGFBP3 | 5′-GACGACGTACATTGCCTCAG-3’ | 5′-GTCTTTTGTGCAAAATAAGGCATA-3’ |
Socs3 | 5′-GCTCCAAAAGCGAGTACCAGC-3’ | 5′-AGTAGAATCCGCTCTCCTGCAG-3’ |
CYP27B1 | 5′-ACCCGACACGGAGACCTTC-3’ | 5′-ATGGTCAACAGCGTGGACAC-3’ |
GAPDH | 5′-AATCCCATCACCATCTTCCA-3’ | 5′-TGGACTCCACGACGTACTCA-3’ |