Table 1 Forward and reverse primers used in the multiplex PCR for Taenia hydatigena, T. multiceps, T. pisiformis, and Dipylidium caninum
Species | Primer names and sequences 5′-3’ | Expected amplicon size (bp) | The location of primers in the mtDNA | Accession no. of mtDNAs in NCBI GenBank |
|---|---|---|---|---|
T. hydatigena | Th-F: AGTTCCATATTATTTACAGTTTTGTTATTAC | 592 | 12,446 | NC_012896 |
Th-R: TAACATAATACTTGAAGACACCCCCA | 13,038 | |||
T. multiceps | Tm-F: GTTGTTGATGTGGCTTAAGTTTTTGTGT | 385 | 10,902 | NC_012894 |
Tm-R: TCTATAAAATAAACACATACACAACAATCCT | 11,287 | |||
T. pisiformis | Tp-F: TGTGGGAAGGTTTAGGTGAATCAT | 283 | 200 | NC_013844 |
Tp-R: GTTAACATCAATATCTTCTAGCTCTGACACT | 483 | |||
D. caninum | Dc-F: CTATTGATTGCGTTTATTGTTTTGTGT | 190 | 8594 | MG587892 |
Dc-R: GAAAAGAAATCAAATACAGTTAAACGGT | 8784 |