Fig. 2

The specificity analysis of the 4 sets of primers using various combined target templates. Lanes 1–4: PCR amplification result of Taenia hydatigena, T. multiceps, T. pisiformis and Dipylidium caninum as single template, respectively; Lanes 5–10: PCR amplification result of Th + Tm, Th + Tp, Th + Dc, Tm + Tp, Tm + Dc and Tp + Dc as dual templates, respectively; Lanes 11–14: PCR amplification result of Th + Tm + Tp, Th + Tm + Dc, Th + Tp + Dc and Tm + Tp + Dc as triple templates, respectively; Lane 15: PCR amplification result of Th + Tm + Tp + Dc as quadruple templates; Lane 16: negative control; Lane M: 2000 bp DNA standard marker