Etiology of childhood diarrhoea among under five children and molecular analysis of antibiotic resistance in isolated enteric bacterial pathogens from a tertiary care hospital, Eastern Odisha, India

Table 1 PCR primers used in the study

Gene	Sequence (5′-3′)	Annealing temp (°C)	Amplicon size (bp)
elt	CACACGGAGCTCCTCAGTC	57	508
elt	CCCCCAGCCTAGCTTAGTTT	57	508
est	GCTAAACCAGTAG/AGGTCTTCAAAA	57	147
est	CCCGGTACAG/AGCAGGATTACAACA	57	147
bfpA	GGAAGTCAAATTCATGGGGG	57	367
bfpA	GGAATCAGACGCAGACTGGT	57	367
AatA	CTGGCGAAAGACTGTATCAT	57	630
AatA	CAATGTATAGAAATCCGCTGTT	57	630
eae	CCCGAATTCGGCACAAGCATAAGC	57	881
eae	CCCGGATCCGTCTCGCCAGTATTCG	57	881
aaiC	ATTGTCCTCAGGCATTTCAC	57	215
aaiC	ACGACACCCCTGATAAACAA	57	215
Stx1	CAACACTGGATGATCTCAG	57	350
Stx1	CCCCCTCAACTGCTAATA	57	350
Stx2	ATCAGTCGTCACTCACTGGT	57	110
Stx2	CTGCTGTCACAGTGACAAA	57	110
bla_CTX-M3	AATCACTGCGTCAGTTCAC	50	701
bla_CTX-M3	TTTATCCCCCACAACCCAG	50	701
NDM-1	ACCGCCTGGACCGATGACCA	58	264
NDM-1	GCCAAAGTTGGGCGCGGTTG	58	264
gyrA	TACACCGGTCAACATTGAGG	64	648
gyrA	TTAATGATTGCCGCCGTCGG	64	648
gyrB	TGAAATGACCCGCCGTAAAGG	64	309
gyrB	GCTGTGATAACGCAGTTTGTCCGGG	64	309
parC	GTCTGAACTGGGCCTGAATGC	64	249
parC	AGCAGCTCGGAATATTTCGACAA	64	249
parE	ATGCGTGCGGCTAAAAAAGTG	64	290
parE	TCGTCGCTGTCAGGATCGATAC	64	290
qnrA	ATTTCTCACGCCAGGATTTG	64	516
qnrA	GATCGGCAAAGGTTAGGTCA	64	516
qnrB	GATCGTGAAAGCCAGAAAGG	64	476
qnrB	ATGAGCAACGATGCCTGGTA	64	476
qnrC	GGGTTGTACATTTATTGAATCG	64	307
qnrC	CACCTACCCATTTATTTTCA	64	307
qnrS	GCAAGTTCATTGAACAGGGT	64	428
qnrS	TCTAAACCGTCGAGTTCGGCG	64	428
aac(6′)-Ib-cr	TTGCGATGCTCTATGAGTGGCTA	55	482
aac(6′)-Ib-cr	CTCGAATGCCTGGCGTGTTT	55	482
qepA	AACTGCTTGAGCCCGTAGAT	55	596
qepA	GTCTACGCCATGGACCTCAC	55	596

ISSN: 1471-2334