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Table 1 Primer sequences and protocols for PCR amplification of complete coding SH gene with flanking non-coding sequence (316 nucleotides) and complete coding HN protein sequences. Nucleotide positions are relative to AF338106 (major component Jeryl-Lynn of live vaccine). The M13 primer binding sites used for sequencing are marked in bold

From: Mumps: MMR vaccination and genetic diversity of mumps virus, 2007–2011 in Catalonia, Spain

Primer NamePrimer Sequence (5′ - 3′)Position (AF338106)
Amplification PCR Protocol
 Outer PCR reactions
  MuV_0FaCAAAACAAATCATATCAAYACAATATCAAG6105–6134
  MuV_0FbGGCTTAYATTGCRACYAAAGA6066–6086
  MuV_0RabTARGAGTATCTCATTTAGGCC8464–8444
  Thermal profile: 45 °C × 30 min - 95 °C ×  15 min - 40 cycles (94 °C ×  30 s - 50 °C ×  1 min - 68 °C ×  3 min) - 68 °C ×  10 min
 Inner PCR reactions
  MuV_SH_1FTGTAAAACGACGGCCAGTTCRAGTAGTGTCGATGATCTCAT6130–6152
  MuV_SH_1RCAGGAAACAGCTATGACCTTRCTCAAGCCTTGRTCATT6810–6791
  MuV_HN_2FTGTAAAACGACGGCCAGTTYCGRACCTGYTTCCGAATA6699–6718
  MuV_HN_2RCAGGAAACAGCTATGACCACTGTTGCAATYGAGCAG7359–7342
  MuV_HN_3FTGTAAAACGACGGCCAGTCATAATGTRATTAATGCCAACTG7196–7218
  MuV_HN_3RCAGGAAACAGCTATGACCCACCAGCTRGTACTYCTCTG7893–7874
  MuV_HN_4FTGTAAAACGACGGCCAGTTGYATTYCTDGTCTGTGCYTG7744–7764
  MuV_HN_4RCAGGAAACAGCTATGACCGCCATTCTGGCCTGTT8442–8427
  Thermal profile: 95 °C × 5 min - 35 cycles (95 °C ×  30 s - 50 °C ×  30 s - 72 °C ×  1 min) - 72 °C ×  10 min
 Sequencing protocol
  M13FTGTAAAACGACGGCCAGT
  M13RCAGGAAACAGCTATGACC
  Thermal profile: 96 °C ×  1 min - 30 cycles (96 °C ×  10 s - 50 °C ×  5 s - 60 °C ×  4 min)