Fig. 1

HBV sequence, repressor transcription activator-like effector (rTALE) cassettes and detection of rTALE-coupled hemagglutinin. a Open reading frames encoded by the HBV sequences, depicted in a linear arrangement, with target sites of SPL and SPR. Approximate location of CpG islands I, II and III are indicated together with transcriptional control elements. S1 PR: Surface 1 promoter: S2 PR: Pre-S2 Promoter; Enh I/X Pr: Enhancer I and X promoter; BCP/Enh II: Basic core promoter and enhancer II; PA: Polyadenylation signal. b Schematic depiction of expression cassettes encoding SPL and SPR rTALEs. A cytomegalovirus (CMV) immediate early promoter/enhancer or modified murine transthyretin receptor (MTTR) regulatory sequence was included to drive transcription of downstream sequences encoding a hemagglutinin tag (HA), Krüppel associated box (KRAB), nuclear localization signal (NLS), DNA-binding TALE and transcriptional termination signal (PA). c Representative fields showing immunofluorescence detection of the HA tag with Alexa Fluor 488-labeled antibodies in liver-derived Huh7 cells that had been transfected with plasmids containing CMV-SPL, CMV-SPR, MTTR-SPL or MTTR-SPR expression cassettes, or an irrelevant sequence (Mock)