Four-color multiplex real-time PCR assay prototype targeting azithromycin resistance mutations in Mycoplasma genitalium

Table 4 Description of samples used in the pre-validation of the M + IC multiplex (Diagenode MGres beta kit)

Mutation E. coli numbering	Comment	Number of samples included in study	Number of samples correctly identified	Sample type
WT	Wild-type	8	8	3 M urethra
				3 M urine
				1 F cervix
				1 F urine
A2059G	Common	9	9	2 M urethra
				2 M urine
				3 F cervix
				1 F urethra
				1 F urine
A2058C	Rare	4	4	4 F cervix
A2058G	Most common	8	8	3 M urethra
				2 M urine
				3 F cervix
A2059T	Very rare	0	ND
A2058T	Relatively rare	9	8^a	3 M urine
				4 F cervix
				2 F urine
A2059C	Rare	2	1^b	1 M urethra
A2059C	Rare	2	1^b	1 F cervix

F female, M male, ^a: one sample not M. genitalium positive (both assays); ^b: one sample with dual populations (one with 2058 mutation and one with 2059 mutation); one misclassified in the sample selection step as it was an A2058C mutation (correctly identified by MGres)

ISSN: 1471-2334