Four-color multiplex real-time PCR assay prototype targeting azithromycin resistance mutations in Mycoplasma genitalium

Table 3 Co-infection interference testing between the plasmidic DNA targets of the M + IC multiplex

Plasmids: 10⁶ copies 5× LoD	Mg219	A2058C	A2058G	A2058T	A2059G	A2059C
Mg219	–	NI / NI	NI / I	NI / I	NI / NI	I / NI
A2058C	NI / NI	–	SD	SD	I* / I*	I* / I*
A2058G	I / NI	SD	–	SD	I* / I*	I* / I*
A2058T	NI / NI	SD	SD	–	I* / I*	I* / I*
A2059G	NI / NI	I* / I*	I* / I*	I* / I*	–	SD
A2059C	NI / NI	I* / I*	I* / I*	I* / I*	SD	–

X / X: in urine / in E-Swab; NI: non interfering; I: interfering; I*: strongly interfering (entirely cancels signals at 5× the LoD); −: same target; SD: same dye. n = 3

ISSN: 1471-2334