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Table 2 Limit of detection for each of the plasmidic DNA targets (Mg219, A2058C, A2058G, A2058T, A2059G, A2059C), with internal control (EIC for urine and ESwab and UIC for Tris-EDTA). N = 6

From: Four-color multiplex real-time PCR assay prototype targeting azithromycin resistance mutations in Mycoplasma genitalium

Target

Urine / EIC

(copies/PCR)

ESwab / EIC

(copies/PCR)

Tris-EDTA / UIC

(copies/PCR)

Mg219

7.30

7.30

6.45

A2058C

23.40

6.45

6.00

A2058G

6.00

6.00

23.40

A2058T

49.85

6.00

6.00

A2059G

36.10

6.45

6.45

A2059C

5.65

6.00

6.00