Table 3 Detection of clinical specimens by multiplex PCR, sequencing method and real-time PCR
From: Development of a multiplex PCR to detect and discriminate porcine circoviruses in clinical specimens
Pig Farm | No. of specimens | multiplex PCR | sequencing method | real-time PCR | Concordance rate (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
PCV1 positive (%) | PCV2 positive (%) | PCV3 positive (%) | PCV1 positive (%) | PCV2 positive (%) | PCV3 positive (%) | PCV1 positive (%) | PCV2 positive (%) | PCV3 positive (%) | |||
1 | 50 | 0 (0) | 36 (72.0) | 33 (66.0) | 0 (0) | 36 (72.0) | 33 (66.0) | 0 (0) | 36 (72.0) | 33 (66.0) | 100 |
2 | 48 | 41 (85.4) | 33 (68.8) | 31 (64.6) | 41 (85.4) | 33 (68.8) | 31 (64.6) | 41 (85.4) | 33 (68.8) | 31 (64.6) | 100 |
3 | 38 | 35 (92.1) | 25 (65.8) | 0 (0) | 35 (92.1) | 25 (65.8) | 0 (0) | 35 (92.1) | 25 (65.8) | 0 (0) | 100 |
4 | 36 | 32 (88.89) | 27 (75.0) | 26 (72.2) | 32 (88.89) | 27 (75.0) | 26 (72.2) | 32 (88.89) | 27 (75.0) | 26 (72.2) | 100 |
5 | 33 | 0 (0) | 21 (63.6) | 20 (60.6) | 0 (0) | 21 (63.6) | 20 (60.6) | 0 (0) | 21 (63.6) | 20 (60.6) | 100 |
6 | 30 | 0 (0) | 17 (56.7) | 19 (63.3) | 0 (0) | 17 (56.7) | 19 (63.3) | 0 (0) | 17 (56.7) | 19 (63.3) | 100 |
7 | 26 | 20 (76.9) | 0 (0) | 0 (0) | 20 (76.9) | 0 (0) | 0 (0) | 20 (76.9) | 0 (0) | 0 (0) | 100 |
8 | 25 | 22 (88.0) | 16 (64.0) | 0 (0) | 22 (88.0) | 16 (64.0) | 0 (0) | 22 (88.0) | 16 (64.0) | 0 (0) | 100 |
Total | 286 | 150 (52.4) | 175 (61.2) | 129 (45.1) | 150 (52.4) | 175 (61.2) | 129 (45.1) | 150 (52.4) | 175 (61.2) | 129 (45.1) | 100 |