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Table 3 Detection of clinical specimens by multiplex PCR, sequencing method and real-time PCR

From: Development of a multiplex PCR to detect and discriminate porcine circoviruses in clinical specimens

Pig Farm No. of specimens multiplex PCR sequencing method real-time PCR Concordance rate (%)
PCV1 positive (%) PCV2 positive (%) PCV3 positive (%) PCV1 positive (%) PCV2 positive (%) PCV3 positive (%) PCV1 positive (%) PCV2 positive (%) PCV3 positive (%)
1 50 0 (0) 36 (72.0) 33 (66.0) 0 (0) 36 (72.0) 33 (66.0) 0 (0) 36 (72.0) 33 (66.0) 100
2 48 41 (85.4) 33 (68.8) 31 (64.6) 41 (85.4) 33 (68.8) 31 (64.6) 41 (85.4) 33 (68.8) 31 (64.6) 100
3 38 35 (92.1) 25 (65.8) 0 (0) 35 (92.1) 25 (65.8) 0 (0) 35 (92.1) 25 (65.8) 0 (0) 100
4 36 32 (88.89) 27 (75.0) 26 (72.2) 32 (88.89) 27 (75.0) 26 (72.2) 32 (88.89) 27 (75.0) 26 (72.2) 100
5 33 0 (0) 21 (63.6) 20 (60.6) 0 (0) 21 (63.6) 20 (60.6) 0 (0) 21 (63.6) 20 (60.6) 100
6 30 0 (0) 17 (56.7) 19 (63.3) 0 (0) 17 (56.7) 19 (63.3) 0 (0) 17 (56.7) 19 (63.3) 100
7 26 20 (76.9) 0 (0) 0 (0) 20 (76.9) 0 (0) 0 (0) 20 (76.9) 0 (0) 0 (0) 100
8 25 22 (88.0) 16 (64.0) 0 (0) 22 (88.0) 16 (64.0) 0 (0) 22 (88.0) 16 (64.0) 0 (0) 100
Total 286 150 (52.4) 175 (61.2) 129 (45.1) 150 (52.4) 175 (61.2) 129 (45.1) 150 (52.4) 175 (61.2) 129 (45.1) 100