Table 1 Sequences of the primers for multiplex PCR
From: Development of a multiplex PCR to detect and discriminate porcine circoviruses in clinical specimens
Primer | Primer sequences (5’-3’) | Origin/target gene | Location | Products |
|---|---|---|---|---|
PCV1-F | GAAAGTGAGCGGGAAGAT | PCV1 (GenBank: KX827790.1) /Rep | 499–516 | 310 bp |
PCV1-R | CTGATTGCTGGTAATCAA | 790–808 | ||
PCV2-F | CACATCGAGAAAGCGAAAGGAAC | PCV2(GenBank: MG229682.1) /Rep | 294–316 | 505 bp |
PCV2-R | TGCGGGCCAAAAAAGGTACAGTT | 776–798 | ||
PCV3-F | AGCAGTGCTCCCCATTGA | PCV3(GenBank: KX898030.1) /Rep, Cap | 1431–1448 | 1021 bp |
PCV3-R | TGGGCCCGACCAAATCCGG | 428–446 |