High incidence of virulence determinants, aminoglycoside and vancomycin resistance in enterococci isolated from hospitalized patients in Northwest Iran

Haghi, Fakhri; Lohrasbi, Vahid; Zeighami, Habib

doi:10.1186/s12879-019-4395-3

Table 1 Primers sequence and annealing tempratures used in this study

From: High incidence of virulence determinants, aminoglycoside and vancomycin resistance in enterococci isolated from hospitalized patients in Northwest Iran

Target	Primer sequence (5′ → 3′)	Amplicon size (bp)	Annealing tempreture	Ref.
ddl _faecalis	ATCAAGTACAGTTAGTCTTTATTAG	941	55 °C	[13]
ddl _faecalis	ACGATTCAAAGCTAACTGAATCAGT	941	55 °C	[13]
ddl _faecium	TTGAGGCAGACCAGATTGACG	658	55 °C	[13]
ddl _faecium	TATGACAGCGACTCCGATTCC	658	55 °C	[13]
van A	CATGAATAGAATAAAAGTTGCAATA	1030 bp	54 °C	[14]
van A	CCCCTTTAACGCTAATACGATCAA	1030 bp	54 °C	[14]
van B	GTGACAAACCGGAGGCGAGGA	433 bp	54 °C	[14]
van B	CCGCCATCCTCCTGCAAAAAA	433 bp	54 °C	[14]
van C	GAAAGACAACAGGAAGACCGC	796 bp	54 °C	[15]
van C	ATCGCATCACAAGCACCAATC	796 bp	54 °C	[15]
aac(6′)-Ie-aph(2″)-Ia	CAGGAATTTATCGAAAATGGTAGAAAAG	369 bp	55 °C	[16]
aac(6′)-Ie-aph(2″)-Ia	CACAATCGACTAAAGAGTACCAATC	369 bp	55 °C	[16]
aph(3′)-IIIa	GGCTAAAATGAGAATATCACCGG	523 bp	55 °C	[16]
aph(3′)-IIIa	CTTTAAAAAATCATACAGCTCGCG	523 bp	55 °C	[16]
ant(4′)-Ia	CAAACTGCTAAATCGGTAGAAGCC	294 bp	55 °C	[16]
ant(4′)-Ia	GGAAAGTTGACCAGACATTACGAACT	294 bp	55 °C	[16]
aph(2″)-Ic	CCACAATGATAATGACTCAGTTCCC	444 bp	55 °C	[16]
aph(2″)-Ic	CCACAGCTTCCGATAGCAAGAG	444 bp	55 °C	[16]
aph(2″)-Ib	CTTGGACGCTGAGATATATGAGCAC	867 bp	55 °C	[16]
aph(2″)-Ib	GTTTGTAGCAATTCAGAAACACCCTT	867 bp	55 °C	[16]
aph(2″)-Id	GTGGTTTTTACAGGAATGCCATC	641 bp	55 °C	[16]
aph(2″)-Id	CCCTCTTCATACCAATCCATATAACC	641 bp	55 °C	[16]
ant(3″)-III	TGATTTGCTGGTTACGGTGAC	284 bp	55 °C	[17]
ant(3″)-III	CGCTATGTTCTCTTGCTTTTG	284 bp	55 °C	[17]
ant(6′)-Ia	ACTGGCTTAATCAATTTGGG	596 bp	55 °C	[17]
ant(6′)-Ia	GCCTTTCCGCCACCTCACCG	596 bp	55 °C	[17]
PAI	GACGCTCCCTTCTTTTGAC	387 bp	54 °C	[18]
PAI	CCAGAGAAATTACTACCAT	387 bp	54 °C	[18]
sprE	GGTAAACCAACCAAGTGAATC	300 bp	56 °C	[18]
sprE	TTCTTCCGATTGACGCAAAA	300 bp	56 °C	[18]
ace	CAGGCCAACATCAAGCAACA	125 bp	58 °C	[18]
ace	GCTTGCCTCGCCTTCTACAA	125 bp	58 °C	[18]
gelE	CGAAGTTGGAAAAGGAGGC	372 bp	54 °C	[18]
gelE	GGTGAAGAAGTTACTCTGA	372 bp	54 °C	[18]
hyl	ACAGAAGAGCTGCAGGAAATG	276 bp	55 °C	[19]
hyl	GACTGACGTCCAAGTTTCCAA	276 bp	55 °C	[19]
cylA	ACTCGGGGATTGATAGGC	688 bp	56 °C	[19]
cylA	GCTGCTAAAGCTGCGCTT	688 bp	56 °C	[19]
esp	TTTGGGGCAACTGGAATAGT	407 bp	56 °C	[18]
esp	CCCAGCAAATAGTCCATCAT	407 bp	56 °C	[18]

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ISSN: 1471-2334

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