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Table 3 Controls for RLEP qPCR and 16S rRNA RT qPCR assays

From: Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples

Control

Purpose

Material/ method

RLEP qPCR

16S rRNA RT qPCR

Negative extraction control

To exclude contamination during extraction procedure

Transport buffer extracted in the same way as samples

NAa

Positive run control

To ensure adequate performance of qPCR

Cloned RLEP plasmid standard

Cloned 16S rRNA plasmid standard

Negative no template control

To exclude contamination during PCR set up

DEPCa treated water

DEPCb treated water

Internal positive control

To exclude false negative results due to inhibition

TaqMan exogenous internal positive control (IPC)c

TaqMan exogenous internal positive control (IPC)c

  1. aNA Not applicable
  2. bDEPC Diethylpyrocarbonate
  3. cApplied Biosystems, Frankfurt, Germany