Skip to main content

Advertisement

Table 3 Controls for RLEP qPCR and 16S rRNA RT qPCR assays

From: Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples

Control Purpose Material/ method
RLEP qPCR 16S rRNA RT qPCR
Negative extraction control To exclude contamination during extraction procedure Transport buffer extracted in the same way as samples NAa
Positive run control To ensure adequate performance of qPCR Cloned RLEP plasmid standard Cloned 16S rRNA plasmid standard
Negative no template control To exclude contamination during PCR set up DEPCa treated water DEPCb treated water
Internal positive control To exclude false negative results due to inhibition TaqMan exogenous internal positive control (IPC)c TaqMan exogenous internal positive control (IPC)c
  1. aNA Not applicable
  2. bDEPC Diethylpyrocarbonate
  3. cApplied Biosystems, Frankfurt, Germany