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Table 1 Samples for technical validation of RLEP qPCR and 16S rRNA RT qPCR

From: Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples

Purpose Sample type (No.) Obtained from Origin
“Must detect RLEP/16S rRNA (DNA)” samples Nasal swab sample (4)a 2 sequencing confirmed MB leprosy patients Togo
PCR standard (1 per RLEP and 16S rRNA qPCR) Cloned RLEP/16S rRNA plasmids with known copy numbers GenExpress, Berlin, Germany
“Must not detect RLEP/16S rRNA (DNA)” samples Nasal swab sample (14)a 7 endemic controls (healthy individuals) Togo
Nasal swab sample (10)a 5 occupational contacts to untreated (MB) leprosy patients Munich, DITMb medical staff
Nasal swab sample (6)a 3 non-exposed healthy controls Munich, DITMb laboratory staff
Swab sample (5)a 5 PCR confirmed Buruli ulcer patients Ghana [24]
Fine needle aspirate (12)a 11 PCR confirmed Buruli ulcer patients Ghana [24]
Swab sample (1)a 3 Patients with PCR confirmed cutaneaous leishmaniasis Munich, accredited diagnostic laboratories of DITMb
Punch biopsy sample (2)a
Mycobacterial culture (13)a M. abscessus, M. africanum, M. avium, M. bovis, M. fortuitum, M. gordonae, M. intracellulare, M. kansasii, M. malmoense, M. marinum, M. microti, M. tuberculosis, M. xenopi National Reference Center for Mycobacteria, Borstel, Germany
Bacterial culture (5)a Microbial flora colonizing human skin or nasal mucosa: Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes and Escherichia coli Max von Pettenkofer-Institute, Ludwig-Maximilians-University, Munich, Germany
  1. a DNA extracts (additional file – protocol 1)
  2. b DITM, Department of Infectious Diseases and Tropical Medicine (accredited according to DIN EN ISO 15189)