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Table 2 Sensitivity and specificity of Pj PCR and betaglucan in serum

From: Serum-based diagnosis of Pneumocystis pneumonia by detection of Pneumocystis jirovecii DNA and 1,3-β-D-glucan in HIV-infected patients: a retrospective case control study

Index tests in serum Sensitivity (%) (95% CI) Specificity (%) (95% CI)
Patients with PCP HIV-infected control patients Blood donors
n = 26 n = 21 n = 18
Pj PCR positive 100 (87–100) 71 (50–86) 100 (87–100)
Pj PCR with Ct ≤34a 96 (79–100) 90 (71–98) 100 (82–100)
BG ≥80 pg/mlb 96 (80–100) 71 (50–86) 100 (82–100)
BG ≥200 pg/mlb 92 (75–99) 86 (65–95) 100 (82–100)
BG ≥400 pg/mlb 88 (70–96) 90 (71–98) 100 (82–100)
Pj PCR positive and BG ≥200 pg/mlb 92 (75–99) 90 (71–98) 100 (82–100)
  1. Pj Pneumocystis jirovecii, PCP Pneumocystis jirovecii pneumonia, CI confidence interval, BG 1,3-ß-D-glucan
  2. aThree PCP patients whose serum samples had been analysed after 1:2 dilution were excluded
  3. bOne PCP patient whose serum sample was not analyzed for BG due to insufficient volume of serum was excluded