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Table 4 Performance of the multiplex RT-LAMP assay and one-step RT-PCR for influenza virus detection using clinical and spiked samples based on qRT-PCR assay

From: Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform

Subtype sample type qRT-PCR
(Ct value)
Positive rate %
(positive number/test number)
RT-LAMP RT-PCR
B clinical 17.1–34.99 100% (35/35) 94% (33/35)
H1N1 clinical/spikeda 19.61–32.6 100%(12/12) 83%(10/12)
H3N2 clinical 21.79–34.89 97%(34b/35) 91%(32/35)
H5N6/H5N8 spiked 22.2–24.54 100%(8/8) 100%(8/8)
H7N9 spiked 21.25 100%(1/1) 100%(1/1)
Total < 35 98.9%(90/91) 92.3%(84/91)
Time required 120 min 60 min 170 minc
  1. Ct cycle threshold
  2. aH1N1: clinical sample (3), spiked sample (9)
  3. b1 sample undetected by RT-LAMP was confirmed with H3N2 by sequencing
  4. cIncluding gel electrophoresis time for confirmation of results