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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Rapid and simple colorimetric detection of multiple influenza viruses infecting humans using a reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) diagnostic platform

Fig. 2

Specificity of influenza RT-LAMP. To evaluate specificity of each RT-LAMP primer set, RT-LAMP was performed to assess cross-reactivity using (a) individual and (b) mixed influenza virus samples. RT-LAMP reactions were conducted by incubation at 65 °C for 1 h. Positive RT-LAMP reactions resulted in a color change from pink to yellow. For confirmation of colorimetric RT-LAMP, see image of agarose gel electrophoresis in Additional file 1: Figure S1. B-Vic: B/Brisbane/60/2008 (Victoria lineage); B-Yam: B/Phuket/3073/2013 (Yamagata lineage); hH1N1: A/California/04/2009; H3N2: A/Perth/16/2009; aH5N1: A/Em/Korea/w149/2006; hH5N6 vac: A/Sichuan/26221/2014; aH5N8: A/Em/Korea/w468/2014; aH5N8 vac: A/gyrfalcon/Washington/41088–6/2014; hH7N9: A/Anhui/1/2013; N.C.: Negative control (D.W.)

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BMC Infectious Diseases

ISSN: 1471-2334

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