Table 2 Oligonucleotides used in the development of the assay
From: Use of nanotechnology for infectious disease diagnostics: application in drug resistant tuberculosis
Probe name | Description | Sequence (5′ to 3′) with modification |
|---|---|---|
CP WT | Wild type probe (strip oligo) | GATCACCAGCGGCATCGAGAAAAAAAAAA -Biotin |
CP ACC | Mutation probe (strip oligo) | GATCACCACCGGCATCGAGAAAAAAAAAA -Biotin |
CP TBD | TB detection probe (strip oligo) | GGC TGT GGG TAG CAG ACC AAAAAAAAAA -Biotin |
CP Control | Control probe (strip oligo) | CCGTTCGACGGT GCA TCT G AAAAAAAAAA -Biotin |
DP TBD | TB detection probe (with AuNP) | HS-AAAAAAAAAACAGATGCACCGTCGAACGG |
DP katG | katG detection probe (with AuNP) | HS-AAAAAAAAAAGCTGGAGCA GATGGGCTT |