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Table 2 Characteristics of studies: test name, reference test, type of evaluation and diagnostic performance

From: Sensitivity and specificity of diagnostic tests for Lassa fever: a systematic review

Author Test Name Reference Test Type of Samples Number of Samples Tested Type of evaluation Sensitivity(95% CI) Specificity(95% CI)
Boisen et al Recombinant Lassa Virus Rapid Diagnostic Test (ReLASV RDT) Nikisins qPCR Serum and plasma 451 Field evaluation 91.2(75.2–97.7) 86(71.4–94.2)
ReLASV Ag ELISA Serum and plasma 451 Field evaluation 94.8(81.3–99.1) 100(88.6–100)
Boisen et al Recombinant Lassa Virus Antigen ELISA(ReLASV Ag ELISA) Nikisins qPCR Serum and plasma 451 Field evaluation 91.2(75.2–97.7) 86(71.4–94.2)
Bausch et al Combined ELISA Antigen/IgM assay Virus Isolation Serum 305 Laboratory 88(77–95) 90(88–91)
Indirect Immunofluorescent antibody test Virus Isolation Serum 305 Laboratory validation 70(57–81) 88(85–90)
Gabriel et al IgM ELISA Standard RT-PCR Serum 270 Laboratory 31.1(25.6–37) 95.7(92.8–97.7)
Recombinant IgM/IgG ELISA Standard RT-PCR Serum 270 Laboratory 25.9(20.8–31.6) 100(98.2–100)
Ibekwe et al IgM ELISA Standard RT-PCR Serum 72 Laboratory 57(41.05–72.95) 77(63.06–90.94)
Meulen et al. IgM/IgG Immunoblot Indirect Immunofluorescent assay(IFA) Serum 157 Laboratory 90.7(84.13–97.27) 96.3(92.2–100)
Emmerich et al Reverse IgG ELISA IFA Serum 643 Laboratory 100(99–100) 99(98–100)
Olschlager et al GPC RT-PCR(Qiagen) Virus isolation Serum and CSF 77 + (1CSF sample) Laboratory 100(99–100)  
Trappier et al Conventional PCR Virus Isolation Serum 195 Laboratory 66(55–77) 78(69.5–84.5)