Skip to main content

Table 4 Distribution of cases on different proportions of A2063G and A2064G tested by ASPCR and compared with nested PCR following with sequencing

From: Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma Pneumoniae

Method   Proportion of mutation (ASPCR) (%) Total
  <S S and < 30 30 and < 50 50 and < 100 100 Negative
Nested PCR + sequencing A2063G 5 14 13 41 33 3 109
WT 10 12 9 12 1 0 44
Negative 5 2 2 1 4 11 25
Total 20 28 24 54 38 14 178
Nested PCR + sequencing A2064G 0 0 1 0 0 0 1
WT 93 55 1 0 0 3 152
Negative 13 1 0 0 0 11 25
Total 106 56 2 0 0 14 178
  1. S: the sensitivity of the ASPCR assay