Table 4 Distribution of cases on different proportions of A2063G and A2064G tested by ASPCR and compared with nested PCR following with sequencing
From: Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma Pneumoniae
Method | Proportion of mutation (ASPCR) (%) | Total | ||||||
|---|---|---|---|---|---|---|---|---|
<S | ≧S and < 30 | ≧30 and < 50 | ≧50 and < 100 | 100 | Negative | |||
Nested PCR + sequencing | A2063G | 5 | 14 | 13 | 41 | 33 | 3 | 109 |
WT | 10 | 12 | 9 | 12 | 1 | 0 | 44 | |
Negative | 5 | 2 | 2 | 1 | 4 | 11 | 25 | |
Total | 20 | 28 | 24 | 54 | 38 | 14 | 178 | |
Nested PCR + sequencing | A2064G | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
WT | 93 | 55 | 1 | 0 | 0 | 3 | 152 | |
Negative | 13 | 1 | 0 | 0 | 0 | 11 | 25 | |
Total | 106 | 56 | 2 | 0 | 0 | 14 | 178 | |