Table 3 Comparison of the performance characteristics of two methods for detection of macrolide resistance mutations at 23S rRNA
From: Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma Pneumoniae
Genotypes | Method | |
|---|---|---|
ASPCR | Nested PCR + sequencing | |
Total | 178 | 178 |
M. pneumoniae positive (no, %) | 164 | 153 |
Resistance mutations | 149 | 110 |
A2063G | 38 | 109 |
A2063G + WT | 53 | 0 |
A2064G + WT | 5 | 0 |
A2063G + A2064G + WT | 43 | 0 |
A2063G + A2064G | 10 | 0 |
A2064G | 0 | 1 |
WT | 15 | 43 |
Negative | 14 | 25 |