Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma Pneumoniae

BMC Infectious Diseases

Table 1 Oligonucleotide sequences and locations on the M129 genome

Name	Sequence (5′-3′)	Position in M129
23S rRNA-F	CTTTCTAATGGAGTTTTTTACTT	119,806—119,828
23S rRNA-R	GCTTGGTGCTTTCCTATTCT	123,068—123,087
23SA2063G-F	GGACGGGAAGACCCCGTGAAGCTTTACT	122,094—122,121
23SA2064G-F	GGACGGAGAGACCCCGTGAAGCTTTACT	122,094—122,121
23S2063/2064-R	CGTTGCGCCTAACGGGTGTCTTCAC	122,069—122,093
NU-F (Np)	TTAGGCGCAACGGGACGG	122,082—122,099
2063MU-F (Msp)	TTAGGCGCAACGGGAIIIG	122,082—122,100
2064MU-F (Msp)	TTAGGCGCAACGGGAIIIAG	122,082—122,101
23S2063/4D-R	CTGGATAACAGTTACCAATTAGAACAGC	122,233—122,260

The target mutations in the primers of sit-directed mutations (23SA2063G-F and 23SA2064G-F) are shown in boldface. The target mutations at the end of the mutant-specific primers (Msp) are shown in boldface and underlined. The internal mismatches in the mutant-specific primers (Msp) are shown in boldface italics

ISSN: 1471-2334