PCR round | Primer name. | Primer Sequence.(5′ → 3′) | Annealing temp (°C) | Positive Control | Allelic Family |
---|---|---|---|---|---|
Primary | 1 | ATGAAGGTAATTAAAACATTGTCTATTATA | 55 | ||
4 | ATATGGCAAAAGATAAAACAAGTGTTGCTG | 55 | |||
Secondary | A1 | GCAGAAAGTAAGCCTTCTACTGGTGCT | 55 | 3D7 | IC3D7 |
A2 | GATTTGTTTCGGCATTATTATGA | 55 | |||
B1 | GCAAATGAAGGTTCTAATACTAATAG | 55 | HB3 | FC27 | |
B1 | GCTTTGGGTCCTTCTTCAGTTGATTC | 55 |