Fig. 3

Expression of MUC15 was positively correlated with inflammatory factors a: Quantitative real-time RT-PCR analysis of IL1B, IL6, IL8, TNF, CCL2, IFNB1, CXCL10, (solid line, left y axis) and MUC15 (dotted line, right y axis) mRNA expression in hNECs infected with H3N2 (n = 11), using PGK1 as the internal control. Relative mRNA expression levels were calculated using 2^ ^(−ΔΔCt) method; the fold change was compared against uninfected control subjects. b: The correlations between mRNA level (2^ ^(−ΔΔCt)) of MUC15 versus IL1B, IL6, IL8, TNF, CCL2, IFNB1, CXCL10 in hNECs infected with H3N2 were analyzed (n = 11). The timepoints post-infection that were included in the graphs are 8, 16, 24, 48 and 72 hpi. *, **, ***, **** denotes P value of less than 0.05, 0.01, 0.001, < 0.0001 compared with uninfected control, respectively. Uni: uninfected control