Table 1 The oligonucleotide primers and probes for real-time PCR used in this study
Organism | Target gene | Primer sequence (5′-3′) | Probe (5′-3′) | Citation |
|---|---|---|---|---|
S. pneumoniae | lytA | F: ACGCAATCTAGCAGATGAAGC R: TGTTTGGTTGGTTATTCGTGC | FAM-TTTGCCGAAAACGCTTGATACAGGG-BHQ | [29] |
H. influenzae | bexA | F: GGCGAAATGGTGCTGGTAA R: GGCCAAGAGATACTCATAGAACGTT | HEX-CACCACTCATCAAACGAATGAGCGTGG- BHQ | [29] |
GBS | cfb | F: AGCTCTATTAGAAGTACATGCT R: CATTTGCTGGGCTTGATTATT | ROX-ATCAAGTGACAACTCCACAAGTGGTAA- BHQ2 | [30] |
E. coli | uid | F: GTGTGATATCTACCCGCTTCGC R: GAGAACGGTTTGTGGTTAATCAGGA | ROX-TCGGCATCCGGTCAGTGGCAGT-BHQ2 | [31] |
N. meningitidis | ctrA | F: GCTGCGGTAGGTGGTTCAA R: TTGTCGCGGATTTGCAACTA | FAM- CATTGCCACGTGTCAGCTGCACAT-BHQ | [32] |
L. monocytogenes | iap | F: CTAAAGCGGGAATCTCCCTT R: CCATTGTCTTGCGCGTTAAT | HEX- CTTCTGGCGCACAATACGCTAGCACT- BHQ | [33] |
mumps virus | Fusion protein | F:TCTCACCCATAGCAGGGAGTTATAT R: GTTAGACTTCGACAGTTTGCAACAA | HEX-AGGCGATTTGTAGCACTGGATGGAACA- BHQ | [34] |
herpes simplex virus | polymerase | F:CATCACCGACCCGGAGAGGGAC R: GGGCCAGGCGCTTGTTGGTGTA | FAM- CCGCCGAACTGAGCAGACACCCGCGC- BHQ | [35] |
Japanese encephalitis virus | polyprotein | F: AGAACGGAAGAYAACCATGACTAAA R: CCGCGTTTCAGCATATTGAT | ROX-ACCAGGAGGGCCCGG- BHQ2 | [36] |
enterovirus | polyprotein | F: CCGGCCCCTGAATGC R: CACCGGATGGCCAATCCA | ROX-AACCGACTACTTTGGGTGTCCGTGTTTC- BHQ2 | [37] |
adenovirus | hexon | F: GCCACGGTGGGGTTTCTAAACTT R: GCCCCAGTGGTCTTACATGCACATC | CY5-TGCACCAGACCCGGGCTCAGGTACTCCGA -BHQ2 | [38] |
varicella zoster virus | DNA polymerase | F: CGGCATGGCCCGTCTAT R: TCGCGTGCTGCGGC | HEX-ATTCAGCAATGGAAACACACGACGCC- BHQ1 | [39] |