The diagnostic value of metagenomic next-generation sequencing for identifying Streptococcus pneumoniae in paediatric bacterial meningitis

Table 2 Comparison of different mNGS unique read groups

Items	mNGS unique reads ≤ 100 (N = 17)	mNGS unique reads > 100 (N = 15)	P
Sex (female/male)	12/5	7/8	0.169
Age (months)	11.2(5.9,33.5)	13.9(9.2,48.9)	0.290
Duration from onset to CSF collection (days)	20(13,35)	11(7,16)	*0.027*
Fever [n (%)]	17(100)	15(100)
Seizure [n (%)]	8(47.1)	10(66.7)	0.265
Vomiting [n (%)]	10(58.8)	9(60)	0.946
Meningeal irritation [n (%)]	9(52.9)	12(80)	0.103
Altered mental status [n (%)]	7(41.2)	8(53.3)	0.492
Peripheral blood
WBC (×10⁹/L)	16.98(15.10,23.69)	17.3(11.9,24,2)	0.895
Neutrophils (%)	78.2(60.7,86.3)	77.8(62.7,85.6)	0.985
Haemoglobin (g/L)	104.4 ± 19.0	105.5 ± 18.7	0.767
C-reactive protein (mg/L)	63.5(19.6104)	109.5(49,157.4)	0.281
1st CSF^a
WBC (×10⁶/L)	1714(495,2635)	1886(910,3600)	0.597
Protein (mg/L)	1280(779,2250)	1650(1161,3033)	0.180
Glucose (mmol/L)	1.84(1.00,3.34)	0.67(0.19,1.92)	0.081
Simultaneous CSF^b
WBC (×10⁶/L)	10 (2,12)	32(14,76)	*0.001*
Protein (mg/L)	1280(779,2250)	855(527,1488)	*0.016*
Glucose (mmol/L)	3.42 ± 0.99	3.39 ± 1.12	0.928

Note: ^aThe first CSF refers to the first cerebrospinal fluid examination in the early stage of the disease
^bSimultaneous CSF refers to the CSF results by clinical testing at which time CSF was also tested by mNGS
P-values in bold italic shows variables with evidence of association in univariate analysis

ISSN: 1471-2334