Fig. 4

Molecular identification of VL cases from the non-program districts of Nepal. Agarose gel electrophoresis showed the sizes of amplified products by nested PCR using primers targeting kintetoplast minicircle DNA of Leishmania spp. Amplified band of the target size (720 bp) were also confirmed to be of Leishmania spp. by Sanger’s sequencing and BLAST. Samples 1 to 9 were collected from VL suspected patients visited to Sukraraj Tropical and Infectious Diseases Hospital (STIDH = STH) from different districts. Two different bands were detected while the band size (approx. 720 bp) is the positive cases for VL (STH6, STH7 and PC). PC for positive control and NC for negative control