Multiplexed kit based on Luminex technology and achievements in synthetic biology discriminates Zika, chikungunya, and dengue viruses in mosquitoes

Table 4 Examples of viral titers in infected mosquito leg tissue

Virus	Mosquito identity	Leg titer, genome equivalents/mL^a
DENV1	Ae. aegypti #168	1.18 × 10⁵
DENV1	Ae. aegypti #169	1 × 10³
CHIKV	Ae. albopictus #11	1.81 × 10⁵
CHIKV	Ae. albopictus #14	0.4 × 10³
CHIKV	Ae. albopictus #15	1.71 × 10⁶
CHIKV	Ae. albopictus #16	6.67 × 10⁵
CHIKV	Ae. albopictus #19	8.78 × 10⁵
ZIKV	Ae. aegypti #16	4.01 × 10⁵
ZIKV	Ae. aegypti #19	3.99 × 10⁶
ZIKV	Ae. aegypti #26	1.98 × 10⁶
ZIKV	Ae. aegypti #57	7.70 × 10⁵
ZIKV	Ae. aegypti #61	6.10 × 10⁵

^aA lower viral titer is present in mosquito legs than in the whole mosquito body, as described for DENV [49] and CHIKV [60, 61]

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