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Table 1 Arboviruses in this study and their viral titer

From: Multiplexed kit based on Luminex technology and achievements in synthetic biology discriminates Zika, chikungunya, and dengue viruses in mosquitoes

Family/Genus

Viruses /strains/GenBank

Viral stocks titers, genome equivalents/mL

Flaviviridae/ Flavivirus Group IV positive ssRNA

DENV1 from Key West (2010), BOL-KW010; GB: JQ675358

1.9 × 108

DENV2, New Guinea C (1944), M29898WSV

2 × 106

DNV3, H-87, (1956), TC00881 WSV

1.6 × 107

DENV4, H-87, (1956), TC00881 WSV

4 × 108

ZIKV, Puerto Rico (2015), strain H/PF/2013; GB: KU501215.1

1.12 × 1010

JEV, Nakayama-GIII (1935) GB: EF531853.1

1 × 1010

YFV, Ghana/Asibi/1927 GB: AY640589

4.57 × 109

MVEV, MVE-1-51, GB: AF161266

2.69 × 107

Togaviridae/Alphavirus Group IV, positive ssRNA

CHIKV, La Reunion (2006), Indian Ocean strain (IOC), LR2006-OPY1, GB: KT449801

1.89 × 108

CHIKV, British Virgin Islands (2013), BVI, Asian lineage; GB: KJ451624

2.4 × 1010

MAYV, strain TRVL 4675 (1954) GB: MK070492.1

3.8 × 107

Peribunyaviridae/ Orthobunyavirus Group V negative ssRNA

CEV, BFS283,

Small segment (S), GB: U12797) Medium segment (M), GB: AF123483

2.69 × 1010

LACV, Original Wisconsin virus (LACV/ human/1960) GB: EF485030–EF485032

4.37 × 109