Table 2 Primers used for amplification and sequencing
From: Nosocomial bloodstream infection and the emerging carbapenem-resistant pathogen Ralstonia insidiosa
Primer | Sequence (5′ → 3′) |
|---|---|
OXA569-up-EcoRI | AACGAATTCATGATGAAACTCCGCCACGC |
OXA569-down-SacI | AACGAGCTCAAATCAGTGACTCGCAAGGGCCA |
OXA570-up-EcoRI | AACGAATTCATGTTTGCTCGCTGGTCAAA |
OXA570-down-SacI | AACGAGCTCAAACTAGGGTGTTGGCCACA |
OXA573-up-EcoRI | AACGAATTCATGTTCCCTCGCTGGTCAAA |
OXA573-down-SacI | AAAGAGCTCAAACTAGGGTGTTGGCCAC |
OXA574-up-EcoRI | AACGAATTCATGATGAAACTCCGCCACGC |
OXA574-down-XhoI | AACCTGCAGAAATCAGTGGCTCGCAAGGGC |
M13 R | GTAAAACGACGGCCAGT |
M13 F | CAGGAAACAGCTATGACC |
OXA570-RT-F | TCAAGCGCACGCCGAGTTGA |
OXA570-RT-R | GGCACCCGTATCGAAGGC |
OXA573-RT-F | ATGACCTCAAGCGCGTGTTC |
OXA573-RT-R | GCACCCGTATCGAAGGC |
RpoB-R.in-F | GTCCATCAGGTTCCCTTCC |
RpoB-R.in-R | GGACAGGTGATACGACACGA |