First isolation and genotyping of Bartonella henselae from a cat living with a patient with cat scratch disease in Southeast Europe

Table 1 Time course of growth of Bartonella spp. and PCR confirmation after blood of the infected cat was inoculated onto agar plates (primary culture) or subcultured (secondary culture)

Plate no.	Agar	Culture	Result after days of incubation
Plate no.	Agar	Culture	3	4	6	7
1	TSA	Primary	Initial growth (SI)		Visible growth	PCR+
1	TSA	Secondary	–	Initial growth	Visible growth	PCR+
2	TSA	Primary	Initial growth (SI)		Visible growth	PCR+
2	TSA	Secondary	–	Initial growth	Visible growth	PCR+
3	BH	Primary	Initial growth (SI)		Visible growth	PCR+
3	BH	Secondary	–	Initial growth	Visible growth	PCR+
4	BH	Primary	Contaminated, discarded	–	–	–
4	BH	–	–	–	–	–

ISSN: 1471-2334