Diversity of HIV-1 genotypes and high prevalence of pretreatment drug resistance in newly diagnosed HIV-infected patients in Shanghai, China

Table 1 List of primers for PCR and sequencing of pol gene in the study

Procedure	Name (direction)	Sequences(5’-3’)	Position^a	Length of target fragment
The first round RT-PCR	MAW 26 (F)	TTGGAAATGTGGAAAGGAAGGAC	2028–2050	1513
The first round RT-PCR	RT21 (R)	CTGTATTTCTGCTATTAAGTCTTTTGATGGG	3539–3509	1513
The second round PCR	PRO-1 (F)	CAGAGCCAACAGCCCCACCA	2147–2166	1316
The second round PCR	RT20 (R)	CTGCCAGTTCTAGCTCTGCTTC	3462–3441	1316
Sequencing	PROS3 (F)	GCCAACAGCCCCACCA	2151–2166	692
	PROC1S (R)	GCTGGGTGTGGTATTCC	2842–2826	692
	RTB (F)	CCTAGTATAAACAATGAG ACAC	2946–2967	511
	RT20S3 (R)	GTTCTAGCTCTGCTTC	3456–3441	511
	RTAS (F)	CTCAGATTGGTTGCAC	2524–2539	Single-Read Sequencing

^aNucleotide positions with reference to the HIV HXB2 strain (Genbank accession number K03455)
F -forward; R-reverse

ISSN: 1471-2334