Fig. 1

Gating strategy for IL-4, IL-10, IFN-γ, IL-17 produced by CD4⁺ and CD8⁺ T cells. T cells were derived from total live PBMCs gated by forward and side scatter followed by single-cell gating using width and height parameters. CD4⁺ and CD8⁺ T cells were defined by the co-expression of CD3 and CD4 or CD8. The above cells were shown in the red boxes as indicated. Percentage of IL-4, IL-10, IFN-γ, IL-17 produced by CD4⁺ and CD8⁺ T cells were further calculated according to the fluorescence of each cytokine antibody