Table 1 The information on primer and probe of RAA assay
Primer/probe | Sequence (5′-3′) | Genomic position | Product size (bp) |
|---|---|---|---|
F-primer | ATTCGCATGCCCCAACCTCAATCACTCACC | 311–342 | 443 |
R-primer | AATACCACATCATCCATATAACTGAAAGCC | 721–751 | |
Probe | TATCATCTTCCTCTTCATCCTGCTGCTATGCCTCA [FAM-DT][THF][BHQ-DT] TCTTGTT GGTTC [C3-spacer] | 391–441 |