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Table 2 Mutations in pncA gene of M. tuberculosis in 51 isolates

From: Pyrazinamide resistance and mutations in pncA among isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan

Nucleotide position Codon Change A.A change Mutation Type TBDReaMDB GMTV Database Reported (literature) No. of isolates
33C > A 11.AAC / AAA uASN11LYS Non-synonymous Unreported Unreported No 1
35A > C 12.GAC/GCC aASP12ALA Non-synonymous reported reported 2
53C > A 18.TCG/TAG SER 18 STOP non-sense Unreported Unreported No 1
56 T > G 19.CTG/CGG uLEU19ARG Non-synonymous reported Unreported 1
137C > T 46.GCA/GTA uALA46VAL Non-synonymous reported reported 1
161C > T 54.CCG/CTG aPRO54LEU Non-synonymous reported reported 1
170A > C 57.CAC/CCC aHIS57PRO Non-synonymous reported Unreported 1
194_203 Del CCTCGTCGTG FRAMSHIPT FRAMSHIPT Unreported Unreported No 1
202 T > C 68.TGG/CGG aTRP68ARG Non-synonymous reported reported 1
205C > A 69.CCA/ACA uPRO69THR Non-synonymous Unreported Unreported No 3
211C > T 71.CAT/TAT aHIS71TYR Non-synonymous reported reported 3
212A > G 71.CAT/ CGT aHIS71ARG Non-synonymous reported reported 1
226A > C 76.ACT/CCT aTHR76PRO Non-synonymous reported reported 1
286A > C 96.AAG/CAG aLYS96GLN Non-synonymous reported Unreported 4
317–18 Del TC uFRAMSHIPT FRAMSHIPT Unreported Unreported No 3
331G > T 111. GAG/TAG STOP Non-sense Unreported Unreported No 1
359 T > G 120. CTG/CGG uLEU120ARG Non-synonymous Unreported reported 1
368G > C 123. CGC/CCC aARG123PRO Non-synonymous Unreported reported 1
376G > A 126. GAT/AAT uASP126ASN Non-synonymous Unreported Unreported No 2
385G > A 129. GAT/AAT uASP129ASN Non-synonymous Unreported Unreported [7] 1
391G > T 131. GTC/TTC uVAL131PHE Non-synonymous Unreported reported 1
398 T > C 133. ATT/ACT aILEU133THR Non-synonymous Unreported reported 2
419G > A 140. CGC/CAC uARG140HIS Non-synonymous Unreported Unreported No 2
422A > C 141. CAG/CCG uGLN141PRO Non-synonymous Unreported reported 3
430G > A 144. GAG/AAG uGLU144LYS Non-synonymous Unreported Unreported No 1
437C > T 146. GCG/GTG aALA146VAL Non-synonymous reported reported 1
449G > C 150. GGC/GCC uGLY150ALA Non-synonymous Unreported Unreported No 1
461G > C 154. AGG/ACG uARG154THR Non-synonymous Unreported Unreported [36] 1
470 T > G 157. GTG/GGG aVAL157GLY Non-synonymous Unreported Unreported [9, 37, 38] 2
508G > C 170. GCC/CCC uALA170PRO Non-synonymous Unreported Unreported No 1
519G > A 173. GAG/GAA uGLU173GLU synonymous Unreported Unreported No 1
522G > A 174. GAG/GAA uGLU173GLU synonymous Unreported Unreported No 1
530 DEL C uFRAMSHIPT FRAMSHIPT Unreported Unreported No 3
535A > T 179. AGC/TGC uSER179CYS Non-synonymous Unreported Unreported [39] 4
535A > G 179. AGC/GGC uSER179GLY Non-synonymous Unreported Unreported No 1
538G > T 180. GTC/TTC uVAL180PHE Non-synonymous reported Unreported 1
  1. TBDReaMDB Tuberculosis Drug Resistance Mutation Database
  2. GMTV, Genome-wide Mycobacterium tuberculosis variation (GMTV): a, Mutations conferring PZAr with very high confidence (category-A). u, mutations with unclear role (category D) (Miotto et al., 2014)