Fig. 1

Design and sensitivity testing of Recombinase Polymerase Assay (RPA) against mef(A) gene. a Schematic of probe and primer design. Taq-Man-style hydrolysis probe is cleaved by nfo endonuclease during amplification, releasing the quencher and activating FAM signal. Quencher serves as 3′ blocking moiety. b RPA sensitivity testing using serial dilutions of DNA from mef(A)-positive Streptococcus pyogenes strain MGAS10394. c Comparison with qPCR using the primers from RPA (b), but using Sybr Green as readout instead of FAM (the probe was not used)