Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid

Table 3 Estimation of diagnostic validity of LF-RPA assay with 114 CSF specimens

		Culture/India ink staining		Performance characteristics (%)
		Positive	Negative	Se	Sp	PPV	NPV
LF-RPA	Pos	40	3	95.2^a	95.8^b	93.0^c	97.2^d
LF-RPA	Neg	2	69	95.2^a	95.8^b	93.0^c	97.2^d
CrAg Lateral Flow Assay	Pos	42	0	100^e	100^f	100	100
CrAg Lateral Flow Assay	Neg	0	72	100^e	100^f	100	100

Pos: positive; Neg: negative; Se: sensitivity; Sp: specificity; PPV: positive predictive value; NPV: negative predictive value; CI: confidence interval.
^a 95% CI: 83.84%-99.42%; ^b 95% CI: 88.30%-99.13%; ^c 95% CI: 81.46%-97.59%; ^d 95% CI: 89.91%-99.26%; ^e 95% CI: 91.59%-100.00%; ^f 95% CI: 95.01%-100.00%.

ISSN: 1471-2334