Fig. 1

Identification of dysregulation in CD4⁺T cell subsets based on the expression of CD127 (IL-7R) and CD25 (IL-2Rα). a Gating strategy for defining subsets of CD4⁺ T cells using CD127 and CD25. Cells were gated based on characteristic light scatter properties FSC against SSC, followed by gating on CD4⁺ T cells. Thereafter based on expression of CD127 and CD25, CD4⁺T cells were further demarcated as naive/memory (CD127⁺CD25^low/−), effector (CD127⁻CD25⁻) and Tregs (CD25^highCD127^low). b Comparison of frequency of CD4⁺ T cells subsets in ART-naïve HIV-1 (n = 21), HIV-2 (n = 16) infected individuals and seronegative individuals (n = 33). Statistical significance was evaluated by unpaired t test; *, p < 0.05; **, p < 0.01; and ***, p < 0.001