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Table 2 Performance characteristics of the in-house real-time duplex PCR assay for the detection of C. trachomatis

From: Comparison of an in-house real-time duplex PCR assay with commercial HOLOGIC® APTIMA assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in urine and extra-genital specimens

Specimen type

COMPARATOR – HOLOGIC® APTIMA assays

Oropharyngeal swab

Ano-rectal swab

Urine

TEST: PCR*

Positive

Negative

Total

Positive

Negative

Total

Positive

Negative

Total

Positive

0

0

0

15

0

15

7

0

7

Negative

0

194

194

0

184

184

0

193

193

Total

0

194

194

15

184

199

7

193

200

Sensitivity

N/A**

100%

100%

Specificity

100%

100%

100%

PPV

N/A**

100%

100%

NPV

100%

100%

100%

% Agreement

100%

100%

100%

  1. *PCR = in-house duplex real-time PCR assay
  2. **N/A = Not applicable, since no C. trachomatis were detected, these values could not be calculated