Table 1 Performance characteristics of the in-house real-time duplex PCR assay for the detection of N. gonorrhoeae
Specimen type | COMPARATOR – HOLOGIC® APTIMA assays | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Oropharyngeal swab | Ano-rectal swab | Urine | |||||||
TEST: PCRa | Positive | Negative | Total | Positive | Negative | Total | Positive | Negative | Total |
Positive | 10 | 0 | 10 | 14 | 0 | 14 | 6 | 0 | 6 |
Negative | 2 | 182 | 184 | 3 | 182 | 185 | 1 | 193 | 194 |
Total | 12 | 182 | 194 | 17 | 182 | 199 | 7 | 193 | 200 |
Sensitivity | 83% | 82.4% | 85.7% | ||||||
Specificity | 100% | 100% | 100% | ||||||
PPV | 100% | 100% | 100% | ||||||
NPV | 98.9% | 98.4% | 99.5% | ||||||
% Agreement | 99% | 98.5% | 99.5% | ||||||