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Table 3 Comparison of qPCR and culture in P. aeruginosa detection in 88 CF sputum samples

From: Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study

CF samples

ecfX-PCR amplificationa

qPCR > CFU count

Culture-positive (n = 41)

41/41

10/41

Culture-negative (n = 47)

9/47

na

  1. athe culture-based counts of CP samples were in the following ranges (CFU/ml): 105–106 (n = 23), 104–105 (n = 9), 103–104 (n = 4), and 102–103 (n = 5); na, not applicable
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BMC Infectious Diseases

ISSN: 1471-2334

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