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Table 2 Intra- and interassay variability of the real-time PCR protocol

From: Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study

Standard dilution

Intra-assay variability

Interassay variability

Mean Cta

CVa (%)

Mean Cta

CVa (%)

10−5 ng/μl

14.18

0.84

14.51

2.2

10−6 ng/μl

17.81

0.23

18.38

3.1

10−7 ng/μl

21.18

0.37

21.31

0.7

10−8 ng/μl

24.78

0.77

24.90

0.9

10−9 ng/μl

27.89

4.0

28.28

1.5

  1. aValues represent the mean threshold cycle (Ct) and coefficient of variation (CV) of three replicates in the same (intra-assay) and in three different (inter-assay) qPCR runs