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Table 2 Intra- and interassay variability of the real-time PCR protocol

From: Detection of viable but non-culturable Pseudomonas aeruginosa in cystic fibrosis by qPCR: a validation study

Standard dilution Intra-assay variability Interassay variability
Mean Cta CVa (%) Mean Cta CVa (%)
10−5 ng/μl 14.18 0.84 14.51 2.2
10−6 ng/μl 17.81 0.23 18.38 3.1
10−7 ng/μl 21.18 0.37 21.31 0.7
10−8 ng/μl 24.78 0.77 24.90 0.9
10−9 ng/μl 27.89 4.0 28.28 1.5
  1. aValues represent the mean threshold cycle (Ct) and coefficient of variation (CV) of three replicates in the same (intra-assay) and in three different (inter-assay) qPCR runs