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Table 1 Laboratory investigations of cerebrospinal fluid (CSF) samples and peripheral blood count of patients with suspected meningitis

From: New molecular tools for meningitis diagnostics in Ethiopia – a necessary step towards improving antimicrobial prescription

 

Microorganism detected

No microorganism detected

All

Virus

Bacteria

Fungi

  

n

(%)

n

(%)

n

(%)

n

(%)

n

(%)

Macroscopic appearance of CSF

 n

21

 

12

 

7

 

2

 

192

 

 Clear

11

(52)

9

(75)

1

(14)

1

(50)

133

(69)

 Bloody

2

(10)

2

(17)

0

(0)

0

(0)

48

(25)

 Turbid

7

(33)

0

(0)

6

(86)

1

(50)

3

(2)

 Xanthochromic

1

(5)

1

(8)

0

(0)

0

(0)

8

(4)

 

n

mean

n

mean

n

mean

n

mean

n

mean

Glucose measurement (mg/mL)

5

27

1

47

4

22

0

 

37

56

Manual cell count using Neubauer chamber

n

(%)

n

(%)

n

(%)

n

(%)

n

(%)

 Total

21

 

12

 

7

 

2

 

180

 

 Not done due to bloody sample

1

(5)

1

(8)

0

(0)

1

(50)

35

(19)

 No cells

5

(24)

5

(42)

0

(0)

0

(0)

94

(52)

 Cell count ≥ 10 cells/mm3

12

(57)

4

(33)

7

(100)

1

(50)

11

(6)

 Mean cells/mm3

 

2740

 

70

 

7716

 

9

 

91

 Median cells/mm3

 

50

 

5

 

2500

 

9

 

0

 Max. cells/mm3

 

40,000

 

332

 

40,000

 

13

 

9

 Min. cells/mm3

 

0

 

0

 

75

 

5

 

0

 

n

mean % PMN

n

mean % PMN

n

mean % PMN

n

mean % PMN

n

mean % PMN

Differential count using Neubauer chamber

14

(30)

6

(0)

7

(60)

1

(0)

45

(3)

Gram staining/India ink staining

n

(%)

n

(%)

n

(%)

n

(%)

n

(%)

 Total

21

 

12

 

7

 

2

 

196

 

 No organisms seen

18

(86)

12

(100)

5

(71)

1

(50)

196

(100)

 Positive

3a

(14)

0

(0)

2a

(29)

1a

(50)

0

(0)

Complete blood count (CBC), peripheral blood

n

mean

n

mean

n

mean

n

mean

n

mean

 White blood count (× 10^9/L)

16

10.5

9

8.3

6

14.7

1

4.7

134

14.2

 Neutrophils (× 10^9/L)

16

6.8

9

3.9

6

11.5

1

3.9

100

7.8

 Lymphocyte count (× 10^9/L)

16

2.4

9

3.1

6

1.8

1

0.3

117

3.5

  1. PMN polymorphonuclear leukocytes
  2. aThe samples positive by Gram strain had Gram-positive cocci (n = 1, CSF positive for Streptococcus pneumoniae on rapid molecular diagnostic (RMD), cell count 2500 (5% lymphocytes, 95% neutrophils), Gram-negative coccobacilli (n = 1, CSF positive for Haemophilus influenzae on RMD, cell count 40,000 with 90% lymphocytes, 10% neutrophils) and fungal elements (n = 1, CSF positive for Cryptococcus neoformans/gattii on RMD, cell count 13 with 100% lymphocytes). All three samples were negative on culture