Fig. 6
From: Motility of human renal cells is disturbed by infection with pathogenic hantaviruses
Effect of N protein expression on podocyte migration capacity. a Motility of podocytes transfected with plasmids encoding for maxGFP and N protein of PUUV or HTNV was analyzed by live cell imaging eight hours after transfection. Cells transfected with pmaxGFP and empty vector served as control (mock). Three independent experiments with 30 cells quantified in each experiment were performed. Shown is mean ± SD. b Transfection efficiency and co-expression of maxGFP and N proteins were monitored by immunostaining for hantaviral N proteins (red). Mean expression level of N proteins was determined by analysis of fluorescence intensity of N protein immunostaining in transfected podocytes. Cells were imaged at a magnification of × 400