Fig. 2

Functional consequences of PUUV-infection in human podocytes. a Podocytes were infected with PUUV at an MOI of 0.5 and viability was assessed on day six post infection. Control cells remained uninfected and viability was set to 100%. Three independent experiments were performed in triplicates. Shown is mean ± SD. b Migration of uninfected and infected podocytes was analyzed by single cell tracking via live cell imaging. Three independent experiments were performed. In each experiment 30 cells were analyzed. Shown is mean ± SD. c For migration assay, podocytes were seeded into μ-plate wells and infected with PUUV at an MOI of 0.5 for six days. After removal of the insert and after eight hours, cell-free areas were measured and relative migration was calculated. Migration of uninfected podocytes was set to 100%. Representative images are shown. Nuclei were stained in blue, N protein in red. Cells were imaged at a magnification of × 100. Three independent experiments were performed. Shown is mean ± SD. d Adhesion of PUUV-infected cells. Uninfected and PUUV-infected podocytes were plated into 96 wells and numbers of attached cells were quantified after one hour. Nuclei were stained in blue, N protein in red. Cells were imaged at a magnification of × 200. Adhesion of uninfected cells was set to 100%. Results were obtained from three independent experiments, each performed in quadruplicate. Shown is mean ± SD