Cross-reactive dengue virus-derived monoclonal antibodies to Zika virus envelope protein: Panacea or Pandora’s box?

Table 2 A summary of hmAb experiments investigating neutralisation of heterologous flavivirus infection

Antibody Name	Target Epitope	Cell line	Neutralisation Measurement	EC_50/PRNT₅₀ Concentration (μg/ml)
1.6D [13]	FLE	LLC-MK2	Focus forming assay	Up to 40
D11C [13]	FLE	LLC-MK2	Focus forming assay	Up to 40
ZKA3 [14]*	EDI/II	Vero	Flow cytometry	0.35
ZKA78 [14]*	EDI/II	Vero	Flow cytometry	0.27
2A10G6 [19]	FLE	BHK-21	PRNT	250
752-2C8 [18]	EDE1	Vero	Focus forming assay	8.9 × 10^− 4
753 (3) C10 [18]	EDE1	Vero	Focus forming assay	3.4 × 10^− 4
B7 [18]	EDE2	Vero	Focus forming assay	Unknown

PRNT Plaque Reduction Neutralisation Test
* = hmAbs taken from ZIKV-infected donors and tested for neutralisation of DENV infection
EC₅₀/PRNT₅₀ concentrations quoted to 2 significant figures

ISSN: 1471-2334