Table 1 Test characteristics
From: Laboratory evaluation of four HIV/syphilis rapid diagnostic tests
| Â | SD Bioline HIV/Syphilis Duo | DPP HIV-Syphilis Assay | Multiplo Rapid TP/HIV Antibody Test | Insti Multiplex HIV-1/HIV-2/Syphilis Antibody Test |
|---|---|---|---|---|
Type of assay | Lateral flow immunochromatographic assay | Lateral flow immunochromatographic assay (dual path platform) | Vertical flow immunofiltration immunoassay (flow through) | Vertical flow immunofiltration immunoassay (flow through) |
Specimen | Serum, plasma, whole blood | Serum, plasma, whole blood | Serum, plasma, whole blood | Serum, plasma, whole blood |
Volume required | 10 μl of serum/plasma | 10 μl of serum/plasma | 1 drop of whole blood/serum/plasma (35-40 μl) | 50 μl of whole blood/serum/plasma |
20 μl of whole blood | 1 sample loop or 10 μl of whole blood | |||
Time to results | 15–20 min | 15–25 min | Once all fluid is absorbed | Immediately after adding the Clarifying Solution |
(+/− 3 min) | ||||
(+/− 1–2 min) | ||||
Equipment | Timer | Timer | None | None |
HIV component | HIV-1/2 recombinant antigens (gp41, gp36 and HIV-1 group O) | HIV-1/2 recombinant antigens (not specified) | HIV-1/2 synthetic peptides (gp36, gp41, gp120 and HIV-1 group O) | HIV-1/2 recombinant antigens (gp41, gp36) |
TP component | Recombinant antigen (17 kDa) | IgG/IgM recombinant antigen (not specified) | IgG/IgM recombinant antigens (15 kDa, 17 kDa, 47 kDa) | Recombinant fusion proteins derived from p17 and p47 domains |
Steps to perform | Add the specimen. | Add the specimen to the Sample Tainer (with buffer), mix. | Add 3 drops of buffer to the device. | Add the specimen to the Sample diluent vial, mix. |
Add 3 drops of diluent. | Add the specimen. | |||
Add 2 drops to the test device at Well 1. | Place the InstantGoldCap. | Add the content of this vial to the device. | ||
Add 4 drops of buffer to the test device at Well 2. | Add 12 drops of buffer. | Add the Colour Developer. | ||
Remove the InstantGoldCap. | Add the Clarifying Solution. | |||
Add 3 drops of buffer. | ||||
Result interpretation | Clear | Clear | Clear | There is no marker on the devices to indicate the position of the HIV, syphilis and control dot. |