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Table 1 Test characteristics

From: Laboratory evaluation of four HIV/syphilis rapid diagnostic tests

  SD Bioline HIV/Syphilis Duo DPP HIV-Syphilis Assay Multiplo Rapid TP/HIV Antibody Test Insti Multiplex HIV-1/HIV-2/Syphilis Antibody Test
Type of assay Lateral flow immunochromatographic assay Lateral flow immunochromatographic assay (dual path platform) Vertical flow immunofiltration immunoassay (flow through) Vertical flow immunofiltration immunoassay (flow through)
Specimen Serum, plasma, whole blood Serum, plasma, whole blood Serum, plasma, whole blood Serum, plasma, whole blood
Volume required 10 μl of serum/plasma 10 μl of serum/plasma 1 drop of whole blood/serum/plasma (35-40 μl) 50 μl of whole blood/serum/plasma
20 μl of whole blood 1 sample loop or 10 μl of whole blood
Time to results 15–20 min 15–25 min Once all fluid is absorbed Immediately after adding the Clarifying Solution
(+/− 3 min)
(+/− 1–2 min)
Equipment Timer Timer None None
HIV component HIV-1/2 recombinant antigens (gp41, gp36 and HIV-1 group O) HIV-1/2 recombinant antigens (not specified) HIV-1/2 synthetic peptides (gp36, gp41, gp120 and HIV-1 group O) HIV-1/2 recombinant antigens (gp41, gp36)
TP component Recombinant antigen (17 kDa) IgG/IgM recombinant antigen (not specified) IgG/IgM recombinant antigens (15 kDa, 17 kDa, 47 kDa) Recombinant fusion proteins derived from p17 and p47 domains
Steps to perform Add the specimen. Add the specimen to the Sample Tainer (with buffer), mix. Add 3 drops of buffer to the device. Add the specimen to the Sample diluent vial, mix.
Add 3 drops of diluent. Add the specimen.
Add 2 drops to the test device at Well 1. Place the InstantGoldCap. Add the content of this vial to the device.
Add 4 drops of buffer to the test device at Well 2. Add 12 drops of buffer. Add the Colour Developer.
Remove the InstantGoldCap. Add the Clarifying Solution.
Add 3 drops of buffer.
Result interpretation Clear Clear Clear There is no marker on the devices to indicate the position of the HIV, syphilis and control dot.