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Table 1 ID results of five suspected strains by four methods

From: Is a positive Christie-Atkinson-Munch-Peterson (CAMP) test sensitive enough for the identification of Streptococcus agalactiae?

Strain numbers Biochemical identification Fluorescent PCR MALDI-TOF MS 16S rDNA identification
Confidence (%) ID results Ct values* ID results Log score values ID results Identity (%) ID results
49 99 GBS 23 (positive) GBS 2.221 GBS 100(6)** GBS
50 98 GBS 28 (grey zone) GBS 2.478 GBS 100(6) GBS
51 98 GBS No Ct No GBS 2.317 GBS 99(6) GBS
53 99 GBS 27 (grey zone) GBS 2.236 GBS 100(6) GBS
54 99 GBS 27 (grey zone) GBS 2.169 GBS 100(6) GBS
ATCC 13813 99 GBS 23 (positive) GBS 2.436 GBS 100(5) & 99(1) GBS
ATCC 19615 99 S.pyogenes No Ct No GBS 2.382 S.pyogenes 100(6) S.pyogenes
ATCC 25923 0 UNIORG*** No Ct No GBS 2.357 S.aureus 100(6) S.aureus
  1. *Positive control: <‚ÄČ23, Negative control: =30 or No Ct
  2. **Figures in brackets denote the number of similar sequences
  3. ***SMIC/ID-2 cards are dedicated to the identification of streptococcus and cannot identify staphylococci