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Table 1 ID results of five suspected strains by four methods

From: Is a positive Christie-Atkinson-Munch-Peterson (CAMP) test sensitive enough for the identification of Streptococcus agalactiae?

Strain numbers

Biochemical identification

Fluorescent PCR

MALDI-TOF MS

16S rDNA identification

Confidence (%)

ID results

Ct values*

ID results

Log score values

ID results

Identity (%)

ID results

49

99

GBS

23 (positive)

GBS

2.221

GBS

100(6)**

GBS

50

98

GBS

28 (grey zone)

GBS

2.478

GBS

100(6)

GBS

51

98

GBS

No Ct

No GBS

2.317

GBS

99(6)

GBS

53

99

GBS

27 (grey zone)

GBS

2.236

GBS

100(6)

GBS

54

99

GBS

27 (grey zone)

GBS

2.169

GBS

100(6)

GBS

ATCC 13813

99

GBS

23 (positive)

GBS

2.436

GBS

100(5) & 99(1)

GBS

ATCC 19615

99

S.pyogenes

No Ct

No GBS

2.382

S.pyogenes

100(6)

S.pyogenes

ATCC 25923

0

UNIORG***

No Ct

No GBS

2.357

S.aureus

100(6)

S.aureus

  1. *Positive control: < 23, Negative control: =30 or No Ct
  2. **Figures in brackets denote the number of similar sequences
  3. ***SMIC/ID-2 cards are dedicated to the identification of streptococcus and cannot identify staphylococci