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Table 2 Detection of HEV RNA by RT-nPCR in feces, serum and tissues of mouse inoculated with capped full-length RNA transcripts of pGEM-HEV or PBS

From: Successful infection of BALB/c mice by a swine hepatitis E virus clone constructed with reverse genetics

Sample

Strand

HEV RNA transcripts

Control

Feces

0 dpi

Positive

–

–

3 dpi

Positive

+(1/3)

+(0/3)

4 dpi

Positive

+(2/3)

+(0/3)

7 dpi

Positive

+(3/3)

+(0/3)

14 dpi

Positive

+(3/3)

+(0/3)

21 dpi

Positive

+(3/3)

+(0/3)

28 dpi

Positive

+(3/3)

+(0/3)

Serum

0 dpi

Positive

+(0/3)

+(0/3)

Negative

+(0/3)

+(0/3)

7 dpi

Positive

+(3/3)

+(0/3)

Negative

+(1/3)

+(0/3)

14 dpi

Positive

+(3/3)

+(0/3)

Negative

+(2/3)

+(0/3)

21 dpi

Positive

+(3/3)

+(0/3)

Negative

+(3/3)

+(0/3)

28 dpi

Positive

+(3/3)

+(0/3)

Negative

+(2/3)

+(0/3)

Tissues

Liver

Positive

+(3/3)

+(0/3)

Negative

+(3/3)

+(0/3)

Spleen

Positive

+(3/3)

+(0/3)

Negative

+(3/3)

+(0/3)

Kidney

Positive

+(2/3)

+(0/3)

Negative

+(2/3)

+(0/3)

Colon

Positive

+(0/3)

+(0/3)

Negative

+(1/3)

+(0/3)