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Table 2 Detection of HEV RNA by RT-nPCR in feces, serum and tissues of mouse inoculated with capped full-length RNA transcripts of pGEM-HEV or PBS

From: Successful infection of BALB/c mice by a swine hepatitis E virus clone constructed with reverse genetics

Sample Strand HEV RNA transcripts Control
Feces 0 dpi Positive
3 dpi Positive +(1/3) +(0/3)
4 dpi Positive +(2/3) +(0/3)
7 dpi Positive +(3/3) +(0/3)
14 dpi Positive +(3/3) +(0/3)
21 dpi Positive +(3/3) +(0/3)
28 dpi Positive +(3/3) +(0/3)
Serum 0 dpi Positive +(0/3) +(0/3)
Negative +(0/3) +(0/3)
7 dpi Positive +(3/3) +(0/3)
Negative +(1/3) +(0/3)
14 dpi Positive +(3/3) +(0/3)
Negative +(2/3) +(0/3)
21 dpi Positive +(3/3) +(0/3)
Negative +(3/3) +(0/3)
28 dpi Positive +(3/3) +(0/3)
Negative +(2/3) +(0/3)
Tissues Liver Positive +(3/3) +(0/3)
Negative +(3/3) +(0/3)
Spleen Positive +(3/3) +(0/3)
Negative +(3/3) +(0/3)
Kidney Positive +(2/3) +(0/3)
Negative +(2/3) +(0/3)
Colon Positive +(0/3) +(0/3)
Negative +(1/3) +(0/3)
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