Fig. 7

Competitive ELISA. PA63 was used to coat ELISA plates. The wells were then incubated with 0.125 μg/mL PA21 and serial dilutions of LF, and the bound antibody was detected by adding peroxidase-conjugated goat anti-human antibody followed by tetramethylbenzidine substrate. OD450 = optical density at 450 nm